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SG-PERT (RT assay)
Why spending a fortune in commercial RT assay or P24 ELISA kits when you can easily quantify viruses with SGPERT?
An easy, robust and inexpensive real-time PCR protocol, which works for any retrovirus.
It's a protocol modified from our original article, in which MS2 RNA replaces BMV RNA (now discontinued)
An easy, robust and inexpensive real-time PCR protocol, which works for any retrovirus.
It's a protocol modified from our original article, in which MS2 RNA replaces BMV RNA (now discontinued)
If you decide to use it for your publication, just be kind and cite us:
Pizzato M, Erlwein O, Bonsall D, Kaye S, Muir D, McClure MO. A one-step SYBR Green I-based product-enhanced reverse transcriptase assay for the quantitation of retroviruses in cell culture supernatants. J Virol Methods. 2009 Mar;156(1-2):1-7. doi: 10.1016/j.jviromet.2008.10.012. Epub 2008 Dec 5. PubMed PMID: 19022294.
Luciferase Assay
When the dark comes to the lab, light up you day with a luciferase assay! Here you can find the recipe for our homemade buffer